{"id":52146,"date":"2022-07-04T10:00:48","date_gmt":"2022-07-04T10:00:48","guid":{"rendered":"https:\/\/stem-toys.gr\/product\/chromatography-kit\/"},"modified":"2022-07-04T10:04:02","modified_gmt":"2022-07-04T10:04:02","slug":"chromatography-kit","status":"publish","type":"product","link":"https:\/\/stem-toys.gr\/en\/product\/chromatography-kit\/","title":{"rendered":"Chromatography Kit"},"content":{"rendered":"<p>Chromatography Kit<\/p>\n<p>Practical paper chromatography offers motivational learning opportunities, but relies on standard analytical grade papers or much less satisfactory alternatives such paper towelling.<\/p>\n<p>Using, for example, a mixture of CMY inkjet printer inks the card will produce a separation column up to 50mm in length in approximately 5 minutes. A whole class set of 100mm long spills can cost less than a single analytical grade equivalent spill<\/p>\n<p>Applications include: basic chromatography separation, forensic techniques, stain diffusion patterning, colour diffusion patterning, records of chemical reactions, fluid syphoning \u2013 i.e., how to empty a cup with a bent wick!<\/p>\n<p><strong>The material is supplied in packs of 10 20x20cm sheets.<\/strong><\/p>\n<h3><b>Paper chromatography<\/b><\/h3>\n<p>It is an\u00a0<a title=\"Analytical chemistry\" href=\"https:\/\/en.wikipedia.org\/wiki\/Analytical_chemistry\">analytical<\/a>\u00a0method used to separate coloured chemicals or substances.<sup id=\"cite_ref-britannica_1-0\" class=\"reference\"><\/sup>\u00a0<a title=\"Erwin Chargaff\" href=\"https:\/\/en.wikipedia.org\/wiki\/Erwin_Chargaff\">Erwin Chargaff<\/a>\u00a0credits in Weintraub\u2019s history of the man the 1944 article by Consden, Gordon and Martin with sparking his discovery of\u00a0<a title=\"Chargaff's rules\" href=\"https:\/\/en.wikipedia.org\/wiki\/Chargaff%27s_rules\">Chargaff\u2019s rules<\/a>, an important precursor to\u00a0<a class=\"mw-redirect\" title=\"Watson and Crick\" href=\"https:\/\/en.wikipedia.org\/wiki\/Watson_and_Crick\">Watson and Crick<\/a>\u2018s discovery of the\u00a0<a class=\"mw-redirect\" title=\"Double-helix structure\" href=\"https:\/\/en.wikipedia.org\/wiki\/Double-helix_structure\">double-helix structure<\/a>\u00a0of\u00a0<a title=\"DNA\" href=\"https:\/\/en.wikipedia.org\/wiki\/DNA\">DNA.<\/a>\u00a0For which they were awarded the\u00a0<a title=\"Nobel Prize in Physiology or Medicine\" href=\"https:\/\/en.wikipedia.org\/wiki\/Nobel_Prize_in_Physiology_or_Medicine\">Nobel Prize in Physiology or Medicine<\/a>\u00a0in 1962.<sup id=\"cite_ref-consden44_3-0\" class=\"reference\"><\/sup><sup id=\"cite_ref-weintraub06_4-0\" class=\"reference\"><\/sup>\u00a0It is now primarily used as a teaching tool, having been replaced in the\u00a0<a title=\"Laboratory\" href=\"https:\/\/en.wikipedia.org\/wiki\/Laboratory\">laboratory<\/a>\u00a0by other chromatography methods such as\u00a0<a title=\"Thin-layer chromatography\" href=\"https:\/\/en.wikipedia.org\/wiki\/Thin-layer_chromatography\">thin-layer chromatography<\/a>\u00a0(TLC).<\/p>\n<p>A paper chromatography variant,\u00a0<a title=\"\" href=\"https:\/\/en.wikipedia.org\/wiki\/Two-dimensional_chromatography\">two-dimensional chromatography<\/a>\u00a0involves using two solvents and rotating the paper 90\u00b0 in between. This is useful for separating complex mixtures of compounds having similar polarity, for example,\u00a0<a class=\"mw-redirect\" title=\"Amino acids\" href=\"https:\/\/en.wikipedia.org\/wiki\/Amino_acids\">amino acids<\/a>. The setup has three components. The mobile phase is a solution that travels up the stationary phase, due to\u00a0<a title=\"Capillary action\" href=\"https:\/\/en.wikipedia.org\/wiki\/Capillary_action\">capillary action<\/a>. The mobile phase is generally a mixture of non-polar organic solvent, while the stationary phase is polar inorganic solvent water. Here paper is used to support the stationary phase, water. Polar water molecules are held inside the void space of the cellulose network of the host paper. The difference between\u00a0<a title=\"Thin-layer chromatography\" href=\"https:\/\/en.wikipedia.org\/wiki\/Thin-layer_chromatography\">TLC<\/a>\u00a0and paper chromatography is that the stationary phase in TLC is a layer of adsorbent (usually\u00a0<a title=\"Silica gel\" href=\"https:\/\/en.wikipedia.org\/wiki\/Silica_gel\">silica gel<\/a>, or\u00a0<a title=\"Aluminium oxide\" href=\"https:\/\/en.wikipedia.org\/wiki\/Aluminium_oxide\">aluminium oxide<\/a>), and the stationary phase in paper chromatography is less absorbent paper.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Chromatography Kit Practical paper chromatography offers motivational learning opportunities, but relies on standard analytical grade papers or much less satisfactory<\/p>\n","protected":false},"featured_media":52145,"template":"","meta":[],"product_brand":[],"product_cat":[315],"product_tag":[],"class_list":{"0":"post-52146","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-technology-replaceables","8":"first","9":"instock","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/product\/52146","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/media\/52145"}],"wp:attachment":[{"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/media?parent=52146"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/product_brand?post=52146"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/product_cat?post=52146"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/stem-toys.gr\/en\/wp-json\/wp\/v2\/product_tag?post=52146"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}